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Soft Tissue Therapy: In addition to spinal adjustments, chiropractors may use soft tissue therapies such as massage, myofascial release, or trigger point therapy to relax tight muscles and reduce tension around the pinched nerve

Immunoblotting Cells were rinsed and pelleted and the pellets were homogenized on ice in a cold Tris-EDTA+0.1% NP-40 lysis buffer

The overnight-grown subcultures were transferred into 96-well plates over 24 h, and their growth curves were measured for cell density (OD 600 ) using a Tecan Spark plate reader (Tecan Group Ltd., Mannedorf, Switzerland)

Garcia L, Garcia F, Llorens F, Unzeta M, Itarte E, Gomez N (2002) PP1/PP2A phosphatases inhibitors okadaic acid and calyculin A block ERK5 activation by growth factors and oxidative stress