glutathione-dependent formaldehyde oxidation pathway Frontiers Deciphering Functions of Intracellular Formaldehyde:

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NRVMs were infected with an optimal amount of adenovirus (determined experimentally to be particle titer MOI 350 to minimize excessive damage to NRVMs while maintaining sufficient expression) as above 48 hours after seeding and media containing penicillin/streptomycin was added on top of the PBS+/+ at the end of the infection

As we age or face heightened environmental exposure (think Torontos winter air pollution and harsh weather), glutathione levels naturally decrease, leading to dullness, uneven skin tone, and more pronounced signs of aging

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At 24 h post-treated, cell viability was determined and 30% inhibitory concentration (IC 30 ) of AFB 1 was calculated