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The protein supernatant was collected by centrifugation at 15,000 rpm at 4 for 10 min and the protein concentrations were measured by bicinchoninic acid (BCA) protein assay kit (Beyotime, China)

Interestingly, the analysis of the tumors by immunoblot revealed decreased levels of galectin-1 and a subsequent elevated levels of granzyme B in tumors that were treated with the combination of sertraline and irradiation ( p = 0.047, p = 0.016, respectively) (Fig