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Cell lysis and immunoblot Histone extracts were prepared using an EpiQuik Total Histone Extraction Kit (EpiGentek, OP-0006-100) according to the manufacturers instructions and quantified using DC Protein Assay Kit (Bio-Rad, 5000111)

Two exercise studies based on blood samples are noteworthy since they tracked intraindividual changes or examined purified exosomes: In the first study, an increase of the pro-inflammatory cytokines IL-6 and IL-1 at 8 h after exercise was predictive of higher post exercise fatigue scores (White et al., 2010) (IL-6 and IL-1 trigger glial reactivity)

Cancer cell niche factors secreted from cancer-associated fibroblast by loss of H3K27me3

Conversely, higher concentrations (5075 M) trigger autophagic cell death through Beclin-1 activation and p53 modulation, independent of caspase pathways (Zhao et al., 2016)