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The sequence of primer pairs used for RT-PCR were listed as below: mCol1A1 forward 5-AGCCGCAAAGAGTCTACATG-3, reverse 5-CTTAGGCCATTGTGTATGCAG-3

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V water = 0.2 mL 80 mg3.2 mg = 5 mL BAC water The KLOW Calculator above solves the fixed-ratio math for other vial sizes and draw volumes