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Forgetting to Equalize Pressure: Researchers struggling to draw an accurate amount of BAC water are often fighting a vacuum inside the vial

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van Bon L, Affandi AJ, Broen J, Christmann RB, Marijnissen RJ, Stawski L, Farina GA, Stifano G, Mathes AL, Cossu M, York M, Collins C, Wenink M, Huijbens R, Hesselstrand R, Saxne T, DiMarzio M, Wuttge D, Agarwal SK, Reveille JD, Assassi S, Mayes M, Deng Y, Drenth JP, de Graaf J, den Heijer M, Kallenberg CG, Bijl M, Loof A, van den Berg WB, Joosten LA, Smith V, de Keyser F, Scorza R, Lunardi C, van Riel PL, Vonk M, van Heerde W, Meller S, Homey B, Beretta L, Roest M, Trojanowska M, Lafyatis R, Radstake TR (2014) Proteome-wide analysis and CXCL4 as a biomarker in systemic sclerosis

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Stored properly in the refrigerator and away from light, a reconstituted vial generally stays usable for several weeks thanks to the benzyl alcohol preservative in bacteriostatic water