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J Biol Chem 285:61186126 Turrens JF (2003) Mitochondrial formation of reactive oxygen species

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A second finding was that during the second round of selection, it took the passage population of each transfer 24 h less than the first-round selection to grow from an initial OD 600 of 0.5 to an early mid-exponential phase (OD 600 of ~15) (data not shown), which could be because the GSH production capacity of the evolved strain nearly counteracted the stressed pressure of Acr of each transfer, leading to a lack of severe growth inhibition