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Description

After checking the quality of the samples (FastQC, v.0.11.7), the files were mapped to the mouse genome (Genome build GRCm38) downloaded from Ensembl using the star aligner (Dobin et al, 2013), version 2.7.4a

10.1038/nn.3554 62 HoL.FerruzziM

After discarding the supernatant, islets were incubated in KRBH containing 0.1% BSA, 1 mM glucose and indicated concentrations of somatostatin (SST-14) or insulin at 37 C for 1 h