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1 Oral contraception is preferred for its beneficial effects on SHBG concentrations

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After measuring protein concentrations using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific), the homogenates (20 g total protein) were separated on 12% SDSPAGE gels and transferred to polyvinylidene fluoride membranes

After discovering that excessive acid ceramidase drives cell sensitivity to ferroptosis, the researchers removed the enzyme from senescent and non-senescent cells to see whether its absence would make cells more resilient to induced ferroptosis

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