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Nine CpG dinucleotides were selected from the GSTA4 promoter region for DNA methylation pyrosequencing

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Research applications include IRR binding affinity characterisation, inflammatory cytokine pathway modulation studies in cell culture models, cytoprotective signaling cascade investigation in metabolic and neuronal cell systems, and comparative receptor selectivity profiling across erythropoietin receptor family members

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Additionally, in order to explore the mechanism of action being neuronal apoptosis, the research team assessed levels of neuroinflammation and glial activation by detecting levels of IL-1-beta, IL-10, and TNF-alpha in the brain