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Quantitative real-time reverse transcriptase polymerase chain reaction of ACSL4 Total RNA of liver tissue was extracted using a total RNA purification kits (Thermo Scientific GeneJET RNA Purification Kit, USA) following the manufacturers protocol (Boom et al

All adult patients give written informed consent to anonymous data collection and analysis for research purposes

The plastidial localization of SufC was confirmed by microscopic analysis of tobacco leaves expressing the AtSufC-YFP fusion protein (Xu and Mller, 2004)

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