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OS is a crucial mechanism that contributes significantly to liver damage in MAFLD, playing a critical role in the transition from simple steatosis to NASH

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Viable hepatocytes were resuspended in Hepatocyte Medium (Williams E, 5% FBS, 10 nM insulin, 100 nM dexamethasone, 100 nM triiodothyronine, and 100 g/mL penicillin-streptomycin), or, for Mpc fl/fl and liver-specific knockout ( Mpc2 LKO ) hepatocytes, in high glucose DMEM, 10% FBS, penicillin-streptomycin, and 0.5 g/ml amphotericin B, and plated on collagen-coated tissue culture plates

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