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Cell viability assays The cells were plated in triplicate in 96-well plates (typically 7000 senescent and 2000 non-senescent cells)

In addition, administration of BPC 157 to mice led to attenuation of expression of various pro-inflammatory markers, such as IL-6 and TNF-alpha

This was demonstrated by the fact that administration of the peptide counteracted 5-HT receptor-dependent symptoms induced by pargyline + L-tryptophan, i.e., hyperthermia and wet dog shake related to 5-HT2A agonism, but not 5-HT1A-related forepaw treading, hind limb abduction, or hypothermia