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The H-ESI source was operated in positive mode at spray voltage 3,500 or negative mode at spray voltage 2,500 with the following parameters: sheath gas 35 au, aux gas 7 au, sweep gas 0 au, ion transfer tube temperature 320 C, vaporizer temperature 275 C, mass range 70 to 800 m/z, full scan MS1 mass resolution of 120,000 FWHM, RF lens at 70%, and standard automatic gain control (AGC)

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The number of glycine capsules dispensed and returned was carefully recorded, and compliance was calculated as the percentage of glycine consumed over the glycine supplements prescribed

2.11 Statistical analysis All data were expressed as the mean SD