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Western blotting was performed according to a previously established protocol

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Figure 4 6.3 Future technologies oriented by gutbone axis Beyond referring to the past and taking stock of the present to achieve anti-osteoporosis effects through the gutbone axis, it is equally of great significance to optimize tools for future technologies and to address the issues that exist along the way

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Motor function and strength tests, spontaneous alternation in the Y maze, sociability, Barnes maze trials, and visual cliff tests were analysed manually