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The sample was then cooled on ice and centrifuged at 10,000 g at 25 C for 10 min

Adipose Tissue Biology and Body Composition Laboratory studies investigate effects on adipose tissue: Adipocyte Metabolism: Examination of lipolysis, lipogenesis, mitochondrial function in adipocytes White Adipose Browning: Research on beige adipocyte formation, UCP1 expression in white depots Adipose NNMT: Investigation of visceral adipose NNMT expression and inhibition effects Adipokine Secretion: Studies on leptin, adiponectin, inflammatory cytokine expression Body Composition: Examination of fat mass, lean mass changes with chronic NNMT inhibition Adipose Inflammation: Research on macrophage infiltration, inflammatory signaling Experimental models include adipocyte cell cultures, adipose tissue explants, and body composition assessment in obesity models