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Clemmensen, C

An important barrier for the humoral communication between the gut and the brain is the bloodbrain barrier (BBB), and the significance of gut microbiota for BBB development has recently been shown in germ-free mice, who develop an increased BBB permeability which is restored by the recolonization of microbiota (Vagnerov et al., 2019

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In some experiments, isolated hepatocytes from WT mice were cultured with rIL-1 (10 ng/ml) or rIL-1ra (40 ng/ml) for 6 h, and subjected to the following analyses