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Cells were lysed by two passes through a MicroFluidizer at 10,000 psi

The discovery of 5-FU up-regulation of GPX4 in this study is consistent with the latest reports, and the reproducibility of the results was further confirmed ( Here, we showed that GPX4 inhibition is a promising strategy to selectively eradicate residual CRC cell reservoirs

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The systemic inhibition of GSH synthesis by L-buthionine-S-R-sulfoximine (BSO, Meister, 1995) in liver and kidneys or the exposure to a toxic metalloid such as arsenite, rose brain GSH pools in mice

This destructive process progresses over several years such that at diagnosis approximately only 10-20% of -cell mass remains ( + cells

GST catalyzes the GSH-mediated hydrolysis of sulfonamide bonds, releasing GST competitive inhibitors such as benzylsulfonyl-GSH or S -benzyl-GSH (Koeplinger et al., 1999