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Moreover, zinc deficiency alters cytokine-mediated signaling, enhancing B cell proliferation in response to IL-6 and IL-2 but reducing it following IL-4 stimulation

Western blot, RT-qPCR, CCK-8, and cell cycle assay were applied to evaluate the expression level and proliferation

Also, a similar protocol was successful in the therapy of the other fistulas, that is, gastrocutaneous (Skorjanec et al., 2009), esophagocutaneous (Cesarec et al., 2013), duodenocutaneous (Skorjanec et al., 2015), vesicovaginal (Grgic et al., 2016), and rectovaginal (Baric et al., 2016) in rats

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