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Western Blot Analysis The mouse skin tissues and whole-cell extracts were prepared in RIPA lysis buffer for Western blotting (Cat# P0013, Beyotime, China) and centrifuged at 13700 g for 15 min at 4C

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Expression of Ki-67, cyclin D1 and apoptosis markers correlated with survival in prostate cancer patients treated by radical prostatectomy

Trials 90 , 105892 (2020)

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They demonstrated that palmitoylation of PD-1, but not PD-L1, promoted mTOR signaling and tumor cell proliferation