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Interleukin-17A induces cathepsin K and MMP-9 expression in osteoclasts via celecoxib-blocked prostaglandin E2 in osteoblasts

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Next, 50 ul of specific antibody-alkaline phosphatase conjugate, at a concentration of 25-400 ng, is added and incubated for 2 hours at room temperature

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The samples were sectioned using the Leica CM1850 cryostat (Leica Biosystems, Nussloch, Germany), in 10 m semi-serial sections and adhered to slides prepared with poly-l-lysine solution (SigmaAldrich Co., Missouri, USA)