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(major emphasis is Phase I metabolism very thorough coverage) Smith, Glucuronidation, UNC Chapel Hill, 9/2011 Conjugation Reactions Some Definitions Glucuronidation conjugation is the addition of a molecule to the drug or xenobiotic GSH conjugation GSH = glutathione (a tripeptide derivative) Sulfate conjugation Amino acid conjugation Acetylation Methylation Phase II metabolism is terminology coined by RT Williams, whereby a compound is first subject to oxidation reduction or hydrolysis (Phase I reactions), which may be associated with bioactivation, and then the functional group created is conjugated to a less toxic or inactive compound

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NA cells were pretreated for 1 h with or without NAC (1mM) and then exposed to MNNG (1g/ml) for an additional 24 h

Methylene Blue What it is: Once just a medical dye, methylene blue (MB) is now stealing the spotlight as a mitochondrial multitasker