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Cycling conditions: (1) 95 C/30 s (2) 95 C/10 s (3) 61 C/30 s (4) 72 C/5 min (5) Return to Step 2 for 34 cycles (6) 72 C/5 min

The Co-N coordination bond between cobalt and DMB is, however, not strong, and the nucleotide base can dissociate depending on: (a) pH, (b) temperature, (c) protein environment, and (d) oxidation state of the Co-ion

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It utilized inducible cell lines for initial validation, corroborated relevance in ex vivo and knockout models, and finally interrogated the underlying mechanisms using brain slice electrophysiology and pharmacology

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