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[DOI] [PMC free article] [PubMed] [Google Scholar] 1393.Xia Y, Zweier JL

In sepsis- induced ALI, activated platelets can promote the formation of tissue factor-rich NETs, which in turn can release thrombotic signals (182)

The procedure involved extracting blood samples from filter paper using methanol, followed by drying the supernatant under nitrogen at 40 C, derivatization with butanolic-HCl, and reconstitution with 200 L of acetonitrile-water (50:50 v/v) containing 0.05 % formic acid to adjust pH to 4 for LC-MS/MS analysis