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Finally, the tissue sections were washed thoroughly with PBS, counterstained with DAPI and imaged under a laser scanning confocal microscope (FV-1000, Olympus, Japan)

In aquafeeds, glutamine is typically supplemented as L-glutamine, the bioavailable and stable free form used to meet increased demands under stress

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The word chelate comes from the Greek root chele, which means to claw. EDTAs structure is claw-like and traps heavy metals such as lead, cadmium, mercury, and other toxins from the bloodstream

Our study also highlighted the necessity of future inquiries into other putatively noxious ingredients in UPFs, such as synthetic colorants, emulsifiers, and preservatives, to fully clarify the contributory role of UPFs in escalating allergic conditions and to ascertain their adverse impacts on human health

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