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Inspired by the nuclear envelope localization of zDHHC4, we engineered zDHHCs that ectopically target the ONM to detect mislocalized substrates

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During physical activity, TFEB translocates to the nucleus of skeletal muscle cells and regulates glucose uptake and glycogen content by controlling the expression of glucose transporters (e.g., GLUT4), glycolytic enzymes (e.g., hexokinase), and pathways related to glucose homeostasis (e.g., nitric oxide synthetase)