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Furthermore, there was no significant difference in cellular recycling between Tanzeum and GLP-1 WT, while the effect of L585 truncation was apparent, with 2-fold less of GLP-1 LX being recycled compared to the WT counterpart (Fig

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The AAR was excised from the heart and disrupted in 2 ml of ice-cold isolation buffer (250 mM sucrose, 10 mM HEPES, and 1 mM EGTA, pH 7.4) using a TissueRuptor 22