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To investigate LOD and LOQ of the analytes of interest, a proxy matrix (0.9% NaCl in 10 mmol/L phosphate buffer, pH 7.8) was spiked with decreasing concentrations of the standard solution of N-Hcy-Lys and low-molecular-mass thiols and subsequently subjected to all steps of the analytical procedure

As early as 1940, Brewer showed that the absence of trace oxygen was not sufficient for growing strict anaerobes and developed a medium based on sodium thioglycollate, D-glucose (dextrose) and agar 18

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