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Quantitative real-time PCR (qRT-PCR) was performed using the BeyoFast SYBR Green One-Step qRT-PCR Kit (Beyotime, China) on an MX3000P Real-time PCR system (Stratagene, Germany)

Its relevance to neuropathy is most direct in the metabolic neuropathies, particularly diabetic peripheral neuropathy, where impaired glucose metabolism, insulin resistance, and mitochondrial dysfunction all contribute to nerve damage

Aging 32 , 12621272 (2011)

Enhancing the efficiency and specificity of a genetic vaccine nucleic acid uptake by a given cell type by coating the nucleic acid with an evolved protein that binds to the genetic vaccine nucleic acid, and is also capable of binding to the target cell In one embodiment, the invention provides methods of enhancing the efficiency and specificity of a genetic vaccine nucleic acid uptake by a given cell type by coating the nucleic acid with an evolved protein that binds to the genetic vaccine nucleic acid, and is also capable of binding to the target cell

All mice were kept in a daily cycle of 12 h of light and 12 h of darkness with humidity and temperature controls

These systems are summarized here and their implications are discussed