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Cryo-EM sample preparation and data collection To prepare the apo-form ABCC2 sample, 3.5 L of purified ABCC2 at a concentration of ~9 mg/ml was applied to freshly glow-discharged QUANTIFOIL (R1.2/1.3, 300 mesh, holey carbon films) copper grids

CellTak (Corning) was used to adhere 300,000 live cells per well in a Seahorse 96-well-plate (Agilent)

APOE, primarily synthesized by astrocytes and secreted as HDL-like particles, facilitates lipid trafficking via low-density lipoprotein receptor (LDLR)-mediated uptake

It is studied in controlled models only

Such dynamic tumor-microenvironment interplay drives metastatic coevolution through bidirectional molecular signaling

A.BrennerH.et al (2017)