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M.BaijalR.et al (2013)

Dietary intake of branched-chain amino acids and survival after colorectal cancer diagnosis

A person can have a 500 cal a day diet and their body does not respond negatively

Cell viability assay For cell viability assays, FASN +/+ -PyMT MEFs and HC11 cells were seeded (5 10 3 cells/well) in 96-well plates for 24 h and subsequently replaced with fresh medium with vehicle (control) or increasing concentration of sodium citrate (Sigma) (1, 2.5, 5.0, and 10 mM) or SB204990 (Tocris Bioscience) (10, 20, 30, 40, 60, and 100 M)

The IC50 of cisplatin in these cells was 6.6 M, indicating that these photoactivable iridium compounds could be excellent drugs for targeted therapy [154]

doi: 10.1200/JCO.1995.13.1.26