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If a hydrophobic spacer (acetyl) is used, then Val-Cit has a higher localized DAR than Val-Ala, and if a small amount of linear PEG12 is added, the difference will not be significant

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DTNB (Sigma-Aldrich) was dissolved in 10 PBS (pH 7.4) to make a 4 mg ml 1 (10 mM) solution and subsequently diluted in assay buffer to a final concentration of 0.2 mM