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doi: 10.3389/fnmol.2021.771911 121

In a controlled study, variation in ischemia/reperfusion tolerance across time of day has been previously explored, showing an increase in infarct volume and fibrosis at the onset of activity (ZT12) compared to the beginning of the inactive period (ZT0) in WT mice (Durgan et al., 2010)

The limited efficacy of traditional medications, combined with the high failure rates in clinical drug development due to insufficient efficacy or adverse effects, has raised the bar for the development of the next generation of AD drugs

Hardeland R, Poeggeler B

Coverslips were washed intensively with blocking buffer and then incubated with the adequate Alexa-488, Alexa-594 or Cy5-conjugated secondary antibodies for 45 min at RT

It is critically involved in maintaining cellular homeostasis, mitigating oxidative stress, promoting cell survival and growth, and modulating cell death and autophagy 40