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After 24 hours of incubation, cells that had migrated to the lower surface of the membrane were fixed with 4% paraformaldehyde for 1520 min and stained with crystal violet for 2030 min

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J Thyroid Res 2011(1):402320 Davies TF, Andersen S, Latif R, Nagayama Y, Barbesino G, Brito M, Eckstein AK, Stagnaro-Green A, Kahaly GJ (2020) Graves disease

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Mitochondrial Function Enhances mitochondrial efficiency and ATP production while reducing oxidative stress, supporting cellular energy generation and protecting against age-related mitochondrial decline