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Treatment of cells Hepatoma HepG2 cells were maintained in DMEM (GE Healthcare, Buckinghamshire, UK) supplemented with 10% fetal bovine serum (FBS), 100 U/ml penicillin and 100 g/ml streptomycin at 37 C in 5% CO 2

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As shown in Figure 2B, the addition of an increasing amount of free NS5-RdRp resulted in a dramatic reduction of the ELISA signal as a consequence of the competition with the coated NS5 in interacting with NS3