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4.7 CellTiter-Glo assay Cell viability by loss in ATP content was measured using the CellTiter-Glo Luminescent and CellTiter-Glo 3D Luminescent Cell Viability Assays (Promega)

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MitoSOX Red dye (4 M) or PI (1 g/mL) were added together with Hoechst nuclear counterstain (1 g/mL) (Bio-Sciences) to the cells in DPBS Ca 2+ /Mg 2+ and incubated for 20 minutes at 37 C protected from light