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Each freeze-thaw event introduces micro-crystallisation stress on the protein structure, and repeated cycles accumulate damage that accelerates degradation once the peptide is reconstituted

Repeated dosing studies in rats, dogs, and mice for up to 6 weeks across doses from 0.006 mg/kg to 20 mg/kg via multiple routes showed excellent tolerance

Leuk Res 35:579584 Sargazi S, Sheervalilou R, Rokni M, Shirvaliloo M, Shahraki O, Rezaei N (2021) The role of autophagy in controlling SARS-CoV-2 infection: An overview on virophagy-mediated molecular drug targets

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It has been extensively studied in preclinical and laboratory research for its role in cellular communication, tissue remodeling, and regenerative biochemistry