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The microtiter plate was pre-coated with a GABRA5-specific antibody

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We are also revising 42 CFR 412.3(d)(2) for clarity

Plasmid and siRNA transfection The cells were transfected with plasmids or siRNA for STAT3 or GSTP1, respectively, with Lipofectamine 3000, as per the manufacturers instructions (Thermo Fisher Scientific, Waltham, USA)