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Consistent with perturbed glutathione metabolism, CLP mice exhibited significant downregulation of the Nrf2/HO-1 antioxidant axis: reduced Nrf2, a master transcriptional regulator of antioxidant genes, and its target HO-1 were reduced, indicating impaired upstream antioxidant regulation

ISO 17025 accreditation is an important standard for laboratory competence, testing accuracy, method validation, traceability, and reliable analytical data

KO-K: Writing review & editing, Writing original draft, Supervision, Formal analysis, Conceptualization

Whether combinations produce additive or synergistic effects across the gene-expression pathways modulated by GHK-Cu remains an open research question [12]

doi: 10.3389/fimmu.2021.733808 217 NanWYinJHaoWMengHWuJYinXet al

P-nitroaniline (Sigma-Aldrich, 185310) was used to generate a standard curve (040 nmoles) for the calculation of GGT activity