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Indirect astrocyte state transitions: astrocytes are the modulator of neuronal plasticity It is well established that astrocytes modulate neuronal plasticity indirectly by secreting synaptogenic and anti-synaptogenic cues

doi:10.3109/13697137.2014.929649

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The next day, media were removed from plates, and cells were incubated with 90 L of assay buffer (1 HBSS +20 mM HEPES) + 1% Glosensor substrate for 2 h in the dark at room temperature