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HSCs are regarded as the primary target cells for inflammatory stimuli, and produce extracellular matrix components

Equal amounts of proteins from lysates were separated by SDSPAGE using Mini-PROTEAN TGX 10% Precast Gels (Bio-Rad, Hercules, CA, USA) before transfer onto a Sequi-Blot PVDF membrane (Bio-Rad) using the Mini Trans-Blot Electrophoretic Transfer Cell system (Bio-Rad)