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A transgenic mouse model with an inducible expression of COX-2 found that this induced expression resulted in several premature aging-related phenotypes and a significantly reduced lifespan, while lung fibroblasts from this model exhibited increased expression of SA--gal (Kim et al., 2016)

10.1073/pnas.67.3.1248 97 OshimaT.AibaH.BabaT.FujitaK.HayashiK.HonjoA.et al (1996)

Li, Z.-D

The developed method was assessed for its linearity, selectivity, sensitivity, precision, and accuracy

However, they face limitations in terms of cost-effectiveness, as they require high production costs and have administration restrictions

The reactions were stopped by diluting tenfold into a urea denaturing buffer (50 mM Tris-Cl, pH 7.5, 150 mM NaCl, 8 M urea)