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For glass ampoules: snap the neck at the score point (use a snap ring or gauze to protect your fingers), insert the drawing-up needle and withdraw the medication

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The concentration of each metabolite was normalized to the protein concentration measured by the Protein Assay Dye Reagent (Bio-Rad) in the corresponding sample

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Figure 4 Figure 5 To test whether the reduction kinetics of roGFP2-iL is affected by GRX, roGFP2-iL was fused to the C-terminus of Hs GRX1 and the catalytic efficiency for the reduction of the resulting fusion protein by GSH was compared to free roGFP2-iL in in vitro experiments (Figure 6)

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