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DNA templates for in vitro transcription of sgRNA were generated by annealing a scaffold oligonucleotide sequence: AAAGCACCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTAAAAC, with gene-specific oligonucleotides containing the SP6 promoter sequence (GCGATTTAGGTGACACTATA), a 20-base target sequence (see Table S1), and a complementary sequence to the scaffold oligo (GTTTTAGAGCTAGAAATAG)

Another strategy developed by Arif et al

Semen samples were collected for semen parameter and genetic polymorphism detection, with storage at 80 C designated for research purposes only, not for conception

Rovalpituzumab tesirine, a DLL3-targeted ADC, has shown promising activity in SCLC, particularly among patients with high DLL3 expression (264)

Despite the bright green color of the reagent to the human eye, its use as a contrast agent is based on its robust fluorescence in the NIR range

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