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Activity of PSII photochemical reaction was assayed following the extraction of chloroplast from uniform leaf discs (20 cm diameter) in isolation buffer: 10 mM sodium chloride (NaCl), 10% sucrose, 5% glycerol (v/v), 5 mM magnesium chloride (MgCl 2 )

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Cells for growth and phenotypic assays were grown overnight at 30C (with shaking at 220 rpm) in liquid YPD and were then either harvested or transferred to experiment-specific media for further experimentation