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doi: 10.1016/j.envpol.2013.02.013 45 FormanH

However, when these oxidants accumulate within red cells, hemoglobin and other proteins are oxidized, leading to loss of function and RBC death

Briefly, a RT-PCR-nested amplification was performed, and purified PCR products were quantified using the PicoGreen assay (Invitrogen, Carlsbad, CA, USA), and quality analysed using the BioAnalyzer DNA 1000 LabChip (Agilent, Santa Clara, CA, USA) prior to sequencing