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PROTEIN HANDLING Storing Purified Proteins Purified protein should be filter-sterilized prior to storage

The UPLC system was operated with a gradient elution program consisting of water with 0.1% formic acid (mobile phase A) and methanol (mobile phase B)

Sakhaee K, Poindexter JR, Griffith CS, Pak CY

Mouterde, G., N

Avoid combining with high-percentage L-Ascorbic Acid (Vitamin C) in same routine

Also although this overview focuses on E.coli , protein expression and purification from the other commonly used expression systems are mentioned and apart from cell breakage methods, the protein purification methods and strategies are essentially the same