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The bacterial cells were harvested, resuspended in binding buffer (20 mM sodium phosphate, pH 7.4, containing 500 mM NaCl and 20 mM imidazole), and lysed by treatment as above, followed by sonication

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Learn more about Dipotassium Glycyrrhizate Polyglyceryl-10 Oleate is made by combining ten units of glycerin with oleic acid