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Both RAW 264.7 cells and peritoneal macrophages were maintained in RPMI 1640 medium supplemented with 10% fetal bovine serum, 5% CO, and 95% air at 37C

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( G ) PPAR and PPAR enrichment of the Ppargc1a gene promoter following chromatin immunoprecipitation from cardiac tissue obtained from C57BL/6 mice ( n = 4)

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In contrast, FOXO4-DRI treatment significantly reduced the cell number in the PDL9 group (Supplementary Figures 1, 2)