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There were some limitations to this study

A phase-II clinical study currently underway will evaluate if sulforaphane improves core symptoms in patients with autism spectrum disorders [291]

Lysates were incubated with 80 g of GST-Raf-RBD pre-bound to glutathione-Sepharose in a spin column for 1 hour at 4 C

To test whether RPOA-2 depletion starting at AD6 or later can improve healthspan, burrowing assays were performed at AD10 after nematodes were transferred to rpoa-2 RNAi at AD0, AD6, and AD8